Which one of the followings is not a step in amplification of gene of interest using PCR?

Somatic hybridisation

The correct answer is Option (1) → Somatic hybridisation 

Polymerase Chain Reaction (PCR) is a widely used molecular biology technique that enables the selective amplification of a specific segment of DNA. PCR has become an essential tool in various fields, including genetics, genomics, forensics, and medical diagnostics.

The PCR process involves three main steps:

1. Denaturation

2. Annealing

3. Extension

The polymerase chain reaction (PCR) is a laboratory technique used for the amplification of DNA. It allows the selective and targeted amplification of a specific DNA segment, creating multiple copies of the DNA region of interest. PCR is widely used in various molecular biology applications, including DNA sequencing, genetic analysis, and diagnostics.