Practicing Success

Target Exam

CUET

Subject

Biology

Chapter

Biotechnology and its Applications

Question:

Assertion (A): A single-stranded DNA or RNA probe tagged with a radioactive molecule is used to detect mutated genes.

Reason (R):  The probe will not hybridize with the mutated gene, and the clone with the mutated gene will not appear on the photographic film.

Options:

Both A and R are true, and R is the correct explanation of A.

Both A and R are true, but R is not the correct explanation of A.

A is true, but R is false.

A is false, but R is true.

Correct Answer:

Both A and R are true, and R is the correct explanation of A.

Explanation:

The correct answer is Option (1) -Both A and R are true, and R is the correct explanation of A. 

A single stranded DNA or RNA, tagged with a radioactive molecule (probe) is allowed to hybridise to its complementary DNA in a clone of cells followed by detection using autoradiography. The clone having the mutated gene will hence not appear on the photographic film, because the probe will not have complementarity with the mutated gene.