Target Exam

CUET

Subject

Biology

Chapter

Human Health and Disease

Question:

Read the following passage carefully and answer the given questions.

Immune System in the Body

The human immune system consists of lymphoid organs, tissues, cells and soluble molecules like antibodies. The primary lymphoid organs are bone marrow and thymus where immature lymphocytes differentiate into antigen-sensitive lymphocytes. After maturation the lymphocytes migrate to secondary lymphoid organs like spleen, lymph nodes, tonsils, Peyer's patches of small intestine and appendix. The secondary lymphoid organs provide the sites for interaction of lymphocytes with the antigen, which then proliferate to become effector cells. The bone marrow is the main lymphoid organ where all blood cells including lymphocytes are produced. Each antibody molecule has four peptide chains, two small called light chains and two longer called heavy chains. Hence, an antibody is represented as $H_2L_2$. Different types of antibodies are produced in our body. IgA, IgM, IgE, IgG are some of them. Techniques like radiography (use of X-rays), CT (computed tomography) and MRI (magnetic resonance imaging) are very useful to detect cancers of the internal organs.

Which one of the following technique is not useful for the detection of cancers of internal organs?

Options:

Radiography

CT

DNA fingerprinting

MRI

Correct Answer:

DNA fingerprinting

Explanation:

The correct answer is Option (3) → DNA fingerprinting 

Early detection of cancers is essential as it allows the disease to be treated successfully in many cases. Cancer detection is based on biopsy and histopathological studies of the tissue and blood and bone marrow tests for increased cell counts in the case of leukemias. In biopsy, a piece of the suspected tissue cut into thin sections is stained and examined under microscope (histopathological studies) by a pathologist. Techniques like radiography (use of X-rays), CT (computed tomography) and MRI (magnetic resonance imaging) are very useful to detect cancers of the internal organs. Computed tomography uses X-rays to generate a three-dimensional image of the internals of an object. MRI uses strong magnetic fields and non-ionising radiations to accurately detect pathological and physiological changes in the living tissue. Antibodies against cancer -specific antigens are also used for detection of certain cancers.

DNA fingerprinting involves identifying differences in some specific regions in DNA sequence called as repetitive DNA, because in these sequences, a small stretch of DNA is repeated many times. These repetitive DNA are separated from bulk genomic DNA as different peaks during density gradient centrifugation. The bulk DNA forms a major peak and the other small peaks are referred to as satellite DNA. Since DNA from every tissue (such as blood, hair-follicle, skin, bone, saliva, sperm etc.), from an individual show the same degree of polymorphism, they become very useful identification tool in forensic applications. Further, as the polymorphisms are inheritable from parents to children, DNA fingerprinting is the basis of paternity testing, in case of disputes.