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Pest Resistance Plants

Various nematodes parasitize a wide variety of plants and animals, including human beings. A nematode. Meloidogyne incognitia, infects the roots of tobacco plants and causes a great reduction in yield. A noval strategy was adopted to prevent this infestation which was based on the process of RNA interference (RNAi). RNAi takes place in all eukaryotic organisms as a method of cellular defense. This method involves silencing of a specific mRNA due to a complementary dsRNA molecule that binds to and prevents translation of the mRNA (silencing). Plants, bacteria, fungi and animals whose genes have been altered by manipulation are called Genetically Modified Organisms (GMO). GM plants have been useful in many ways. Genetic modification has: (1) made crops more tolerant to abiotic stresses (cold, drought, salt, heat). (ii) reduced reliance on chemical pesticides (pest- resistant crops). (iii) helped to reduce post harvest losses. (iv) increased efficiency of mineral usage by plants (this prevents early exhaustion of fertility of soil). (v) enhanced nutritional value of food, e.g. golden rice, ie. Vitamin 'A' enriched rice. Genetic modification of organisms can have unpredicatable results when such organisms are introduced into the ecosystem. Therefore, the Indian Government has set up organisations such as GEAC (Genetic Engineering Approval Committee), which will make decisions regarding the validity of GM research and the safety of introducing GM-organisms for public services. Some strains of Bacillus thuringiensis produce proteins that kill certain insects such as lepidopterans (tobacco budworm, armyworm). coleopterans (beetles) and dipterans (flies, mosquitoes).

Bacillus thuringiensis produces proteins that do not kill certain insects such as -

Hymenopterans

The correct answer is Option (3) → Hymenopterans 

Bt toxin is produced by a bacterium called Bacillus thuringiensis (Bt for short). Bt toxin gene has been cloned from the bacteria and been expressed in plants to provide resistance to insects without the need for insecticides; in effect created a bio-pesticide. Examples are Bt cotton, Bt corn, rice, tomato, potato and soyabean etc. Some strains of Bacillus thuringiensis produce proteins that kill certain insects such as lepidopterans (tobacco budworm, armyworm), coleopterans (beetles) and dipterans (flies, mosquitoes). B. thuringiensis forms protein crystals during a particular phase of their growth. These crystals contain a toxic insecticidal protein. Actually, the Bt toxin protein exist as inactive protoxins but once an insect ingest the inactive toxin, it is converted into an active form of toxin due to the alkaline pH of the gut which solubilise the crystals. The activated toxin binds to the surface of midgut epithelial cells and create pores that cause cell swelling and lysis and eventually cause death of the insect.