Which of the following is an important feature required for cloning in a vector?
1. Origin of replication
2. Recognition sites
3. Selectable marker
1. Origin of replication
2. Recognition sites
3. Selectable marker
Answer & explanation
Correct answer: option 4
To facilitate cloning into a vector, following features are required:
1. Origin of replication- a sequence from where replication is initiated
2. selectable marker- the vector requires a selectable marker that helps in identifying and eliminating non-transformants. Normally, the genes encoding for antibiotic resistance genes are considered useful.
3. Cloning sitesS- In order to link the alien DNA, the vector needs to have very few, preferably single recognition sites for the commonly used restriction enzymes.