Practicing Success
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| Which of the following is an important feature required for cloning in a vector? 1. Origin of replication 2. Recognition sites 3. Selectable marker |
1 1, 2 2, 3 1, 2, 3 |
| 1, 2, 3 |
| To facilitate cloning into a vector, following features are required: 1. Origin of replication- a sequence from where replication is initiated 2. selectable marker- the vector requires a selectable marker that helps in identifying and eliminating non-transformants. Normally, the genes encoding for antibiotic resistance genes are considered useful. 3. Cloning sitesS- In order to link the alien DNA, the vector needs to have very few, preferably single recognition sites for the commonly used restriction enzymes. |
