Practicing Success

Target Exam

CUET

Subject

Biology

Chapter

Biotechnology -Principles and Processes

Question:
Which among the following is most widely used in genetic engineering technology ?
Options:
Ribosomes
Plastids
Plasmids
Mitochondria
Correct Answer:
Plasmids
Explanation:
Plasmid is autonomously replicating circular extra-chromosomal DNA. These plasmid DNA act as vectors to transfer the piece of DNA attached to it. A plasmid can be used as vector to deliver an alien piece of DNA into the host organism. The linking of antibiotic resistance gene with the plasmid vector became possible with the enzyme DNA ligase, which acts on cut DNA molecules and joins their ends. This makes a new combination of circular autonomously replicating DNA created in vitro and is known as recombinant DNA.