Which among the following is most widely used in genetic engineering technology ?
Answer & explanation
Correct answer: option 3
Plasmid is autonomously replicating circular
extra-chromosomal DNA. These plasmid DNA act as vectors to
transfer the piece of DNA attached to it. A plasmid can be used as vector to deliver an alien piece
of DNA into the host organism. The linking of antibiotic resistance gene
with the plasmid vector became possible with the enzyme DNA ligase,
which acts on cut DNA molecules and joins their ends. This makes a new
combination of circular autonomously replicating DNA created in vitro
and is known as recombinant DNA.