Which technique generates multiple copies of the DNA?

Polymerase Chain Reaction

The correct answer is Option (2) → Polymerase Chain Reaction

Polymerase Chain Reaction (PCR) is a laboratory technique used to amplify and analyze DNA sequences. It is used to generate multiple copies of a specific DNA sequence. PCR amplifies DNA by cycling through repeated steps of denaturation, annealing, and extension, creating millions of copies from a small DNA sample.It is commonly used for applications such as paternity testing, detection of mutations in genes (including suspected cancer patients), and identifying genetic disorders. PCR is now routinely used to detect HIV in suspected AIDS patients. It is being used to detect mutations in genes in suspected cancer patients too. It is a powerful techqnique to identify many other genetic disorders.

The other options are not used for amplifying DNA:

• Gel electrophoresis: Separates DNA fragments based on size.
• Chromatography: Separates components in a mixture based on their physical or chemical properties.
• DNA ligation: Joins DNA fragments together, but does not amplify DNA.