Read the Passage carefully and answer the questions.
Microbes in human welfare
Microbes are a very important component of life on earth. We use microbes and microbially derived products almost every day, like LAB to make curd, yeast to make bread, adding flavor and texture to cheese, producing antibiotics etc. The addition of LAB, improves the milk quality by increasing vit. B12. Cheese is one of the oldest food items in which microbes are used. For example, large holes in Swiss cheese are due to CO2 produced by the bacterium Propionibacterium sharmanii. The BOD test measures the rate of uptake of oxygen by microorganisms in a sample of water and thus, indirectly, BOD is a measure of the organic matter present in the water. The greater the BOD of waste water, the more is its polluting potential. In anaerobic sludge digesters, bacteria produce a mixture of gases like methane, hydrogen sulphide and carbon dioxide. These gases form biogas and can be used as a source of energy. Biofertilisers are organisms that enrich the nutrient quality of the soil. The main sources of biofertilisers are bacteria, fungi and cyanobacteria. You have studied about the nodules on the roots of leguminous plants formed by the symbiotic association of Rhizobium. These bacteria fix atmospheric nitrogen into organic forms, which is used by the plant as a nutrient. Other bacteria can fix atmospheric nitrogen while free-living in the soil (examples Azospirillum and Azotobacter).
Identify the free living bacteria in soil, which can fix the atmospheric nitrogen.
Answer & explanation
Correct answer: option 3
The correct answer is Option (3) → Azospirillum
Option (1) Rhizobium — Incorrect. Rhizobium does fix nitrogen, but it is symbiotic, not free-living. It lives in the root nodules of leguminous plants and fixes nitrogen there.
Option (2) Glomus — Incorrect. Glomus is a fungus, not a bacterium. It forms mycorrhiza with plant roots and helps the plant absorb phosphorus from the soil. It does not fix nitrogen.
Option (3) Azospirillum — Correct. Azospirillum, like Azotobacter, is a bacterium that fixes atmospheric nitrogen while free-living in the soil. It needs no host plant, and it adds nitrogen to the soil.
Option (4) Anabaena — Incorrect. Anabaena also fixes nitrogen, but it is a cyanobacterium (blue-green alga). It is an autotrophic microbe that makes its own food by photosynthesis and is used as a biofertiliser mainly in paddy fields. NCERT keeps the cyanobacteria as a separate group from the free-living soil bacteria, which are Azospirillum and Azotobacter.
Note 1: The passage is taken from NCERT. NTA has put lines of the NCERT text together to make it. Most CUET (UG) questions come straight from NCERT, so study the book line by line, including figures and their titles.
Note 2: A similar question was also asked in CUET (UG) 2026 on 30-05-2026 (Slot 2), on 29-05-2026 (Slot 2) and on 14-05-2026 (Slot 2), in CUET (UG) 2025 on 29-05-2025 (Slot 1), and in CUET (UG) 2023 on 30-05-2023 (Slot 1).