Match the Column I with Column II :

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Target Exam

CUET

Subject

Biology

Chapter

Biotechnology -Principles and Processes

Question:

Match the Column I with Column II :

Column I	Column II
A. Tumor-inducing (Ti) plasmid	1. Chilled ethanol
B. Restriction endonuclease	2. Removes nucleotides from the ends of DNA
C.Restriction exonuclease	3. Cuts DNA at specific position
D.Isolation of purified DNA	4. Agrobacterium tumifaciens

Options:

A-1,B-2,C-3,D-4

A-3,B-4,C-2,D-1

A-4,B-3,C-2,D-1

A-1,B-3,C-4,D-2

Correct Answer:

A-4,B-3,C-2,D-1

Explanation:

The correct answer is Option (3) -A-4,B-3,C-2,D-1

Column I	Column II
A. Tumor-inducing (Ti) plasmid	4. Agrobacterium tumifaciens
B. Restriction endonuclease	3. Cuts DNA at a specific position
C.Restriction exonuclease	2. Removes nucleotides from the ends of DNA
D.Isolation of purified DNA	1. Chilled ethanol

Explanation:

The tumor inducing (Ti) plasmid of Agrobacterium tumifaciens has now been modified into a cloning vector which is no more pathogenic to the plants but is still able to use the mechanisms to deliver genes of our interest into a variety of plants.
Restriction enzymes belong to a larger class of enzymes called nucleases. These are of two kinds; exonucleases and endonucleases. Exonucleases remove nucleotides from the ends of the DNA whereas, endonucleases make cuts at specific positions within the DNA.
Isolation of purified DNA involves the use of chilled ethanol to precipitate DNA out of a mixture of biomolecules, allowing for the separation and purification of DNA.