Match the Column I with Column II :
| Column I | Column II |
| A. Tumor-inducing (Ti) plasmid | 1. Chilled ethanol |
| B. Restriction endonuclease | 2. Removes nucleotides from the ends of DNA |
| C.Restriction exonuclease | 3. Make cuts at specific positions within the DNA. |
| D.Isolation of purified DNA | 4. Agrobacterium tumifaciens |
Answer & explanation
Correct answer: option 3
The correct answer is Option (3) -A-4,B-3,C-2,D-1
| Column I | Column II |
| A. Tumor-inducing (Ti) plasmid | 4. Agrobacterium tumifaciens |
| B. Restriction endonuclease | 3. Make cuts at specific positions within the DNA. |
| C.Restriction exonuclease | 2. Removes nucleotides from the ends of DNA |
| D.Isolation of purified DNA | 1. Chilled ethanol |
Explanation:
- The tumor inducing (Ti) plasmid of Agrobacterium tumifaciens has now been modified into a cloning vector which is no more pathogenic to the plants but is still able to use the mechanisms to deliver genes of our interest into a variety of plants.
- Restriction enzymes belong to a larger class of enzymes called nucleases. These are of two kinds; exonucleases and endonucleases. Exonucleases remove nucleotides from the ends of the DNA whereas, endonucleases make cuts at specific positions within the DNA.
- Isolation of purified DNA involves the use of chilled ethanol to precipitate DNA out of a mixture of biomolecules, allowing for the separation and purification of DNA.