Match List-I with List-II: 

Choose the correct answer from the options given below:

(A)- (II), (B)- (III), (C) - (IV), (D) - (I)

The correct answer is Option (3) → (A)- (II), (B)- (III), (C) - (IV), (D) - (I)

A. Recombinant DNA technology, Polymerase Chain Reaction (PCR) and Enzyme Linked Immuno-sorbent Assay (ELISA) are some of the techniques that serve the purpose of early diagnosis. ELISA is based on the principle of antigen-antibody interaction. Infection by pathogen can be detected by the presence of antigens (proteins, glycoproteins, etc.) or by detecting the antibodies synthesised against the pathogen.

B. Polymerase Chain Reaction (PCR) is a laboratory technique used to amplify and analyze DNA sequences. It is used to generate multiple copies of a specific DNA sequence. PCR amplifies DNA by cycling through repeated steps of denaturation, annealing, and extension, creating millions of copies from a small DNA sample.It is commonly used for applications such as paternity testing, detection of mutations in genes (including suspected cancer patients), and identifying genetic disorders. 

C. A single stranded DNA or RNA, tagged with a radioactive molecule (probe) is allowed to hybridise to its complementary DNA in a clone of cells followed by detection using autoradiography. The clone having the mutated gene will hence not appear on the photographic film, because the probe will not have complementarity with the mutated gene.

D. Recombinant DNA technology allows scientists to produce human insulin in bacteria (like E. coli) for diabetic patients. Insulin is composed of two peptide chains referred to as the A chain and B chain. A and B chains are linked together by two disulfide bonds, and an additional disulfide is formed within the A chain. In most species, the A chain consists of 21 amino acids and the B chain of 30 amino acids.In 1983, Eli Lilly an American company prepared two DNA sequences corresponding to A and B, chains of human insulin and introduced them in plasmids of E. coli to produce insulin chains. Chains A and B were produced separately, extracted and combined by creating disulfide bonds to form human insulin.