Match List-I with List-II
| List-I | List-II |
|---|---|
| Techniques | Related features |
| (A) ELISA | (I) Production of mature insulin |
| (B) PCR | (II) Antigen-antibody interaction |
| (C) Autoradiography | (III) Amplification of DNA |
| (D) Recombinant DNA technology | (IV) Photographic film |
Choose the correct answer from the options given below:
Answer & explanation
Correct answer: option 3
The correct answer is Option (3) → (A) - (II), (B) - (III), (C) - (IV), (D) - (I)
(A) ELISA — this matches (II) Antigen-antibody interaction. ELISA stands for Enzyme Linked Immuno-Sorbent Assay. It works on the principle of antigen-antibody interaction. It finds an infection by detecting the antigens of the pathogen, such as proteins or glycoproteins, or the antibodies the body has made against it.
(B) PCR — this matches (III) Amplification of DNA. Polymerase Chain Reaction copies a chosen piece of DNA again and again in a test tube. It uses two primers and a heat-stable DNA polymerase. Repeated cycles make about a billion copies, so even a very small amount of a pathogen’s DNA can be detected.
(C) Autoradiography — this matches (IV) Photographic film. A radioactive probe binds to its complementary DNA in a clone of cells. The radiation from the probe darkens a photographic film placed over it, so the clone carrying that gene shows up on the film. A clone with a mutated gene does not bind the probe and does not appear.
(D) Recombinant DNA technology — this matches (I) Production of mature insulin. Human insulin is made of two short chains, A and B, joined by disulphide bridges. In 1983, Eli Lilly put DNA sequences for the two chains into plasmids of E. coli. The bacteria made chains A and B separately, and these were joined to form mature human insulin.