What enzyme is responsible for joining the ends of cut DNA molecules to create recombinant DNA?

DNA ligase

a) DNA ligase

DNA ligase is the enzyme responsible for joining the ends of cut DNA molecules to create recombinant DNA. When a DNA molecule is cut at specific locations by restriction enzymes, there are two loose ends produced. DNA ligase catalyzes the formation of phosphodiester bonds between these loose ends, sealing them together and creating a continuous DNA molecule. This process is essential for the construction of recombinant DNA, where DNA fragments from different sources are joined together to create a new genetic combination.

In the context of genetic engineering, DNA ligase plays a crucial role in the creation of recombinant DNA molecules, which can then be introduced into host organisms for various purposes, such as gene cloning, gene transfer, or the expression of specific genes of interest.