Arrange the following steps which are involved in the process of recombinant DNA technology in correct sequence.
(A) Fragmentation of DNA by restriction endonucleases
(B) Transferring the recombinant DNA into the host
(C) Ligation of desired DNA fragment into a vector
(D) Isolation of DNA
Choose the correct answer from the options given below:
Answer & explanation
Correct answer: option 4
The correct answer is Option (4) → (D), (A), (C), (B)
(D) Isolation of DNA — comes first. The DNA must be pure and free from RNA, proteins and lipids before it can be cut. The cells are broken open with enzymes such as lysozyme, cellulase or chitinase to release it.
(A) Fragmentation of DNA by restriction endonucleases — comes second. The pure DNA of the source and of the vector is cut at specific sites.
(C) Ligation of desired DNA fragment into a vector — comes third. The cut gene and the cut vector are mixed and DNA ligase joins them. This makes the recombinant DNA.
(B) Transferring the recombinant DNA into the host — comes last. The host cell takes up the recombinant DNA, so that it can multiply there.
NCERT Extract:
Processes of Recombinant DNA Technology – …
