The role of interstitial cells in testis is -

The correct answer is Option (2) → Synthesize and secrete testicular hormones

The interstitial cells (Leydig cells) lie in the spaces between the seminiferous tubules, outside the tubules.

(1) Facilitate sperm motility during fertilisation — Incorrect. A sperm swims with its own tail. The interstitial cells have no part in this.

(2) Synthesize and secrete testicular hormones — Correct. Leydig cells make and secrete the testicular hormones called androgens, such as testosterone.

(3) Provide nutrition to dividing germ cells — Incorrect. This is the job of the Sertoli cells, which lie inside the seminiferous tubules. The two cell types are often confused.

(4) Storage and transportation of sperm in testis during coitus — Incorrect. Sperms are stored in the epididymis and carried by the vas deferens. These are ducts, not cells of the testis.

NCERT Extract:
The Male Reproductive System – “… Each seminiferous tubule is lined on its inside by two types of cells called male germ cells (spermatogonia) and Sertoli cells (Figure 2.2 ). The male germ cells undergo meiotic divisions finally leading to sperm formation, while Sertoli cells provide nutrition to the germ cells. The regions outside the seminiferous tubules called interstitial spaces, contain small blood vessels and interstitial cells or Leydig cells (Figure 2.2). Leydig cells synthesise and secrete testicular hormones called androgens.”