Read the passage and answer the following questions :

Once inside the human body, the HIV virus enters macrophages, a type of immune cell. Within the macrophages, the viral RNA genome is replicated to form viral DNA with the assistance of an enzyme called reverse transcriptase. This viral DNA is then integrated into the DNA of the host cell, directing the infected cell to produce virus particles. The infected macrophages continue to produce the virus, acting as HIV factories. Simultaneously, the HIV virus also enters helper T-lymphocytes (TH cells) and replicates within them, producing new viruses. These progeny viruses are released into the bloodstream and target other helper T-lymphocytes, leading to a progressive decrease in their numbers. As the number of helper T-lymphocytes decreases, the infected person experiences various symptoms such as fever, diarrhea, and weight loss. Due to the decrease in helper T-lymphocytes, the person's immune system becomes severely weakened, making them susceptible to infections that could typically be overcome, including those caused by bacteria, such as Mycobacterium, viruses, fungi, and parasites like Toxoplasma. The patient becomes immunodeficient, unable to effectively protect themselves against these infections. Enzyme-linked immunosorbent assay (ELISA) is a widely used diagnostic test for AIDS, allowing for the detection of HIV infection. While antiretroviral drugs can partially prolong the life of AIDS patients, they cannot prevent death, which is inevitable without a cure for the disease. Prevention is crucial in combating AIDS since there is currently no cure. HIV infection primarily spreads through conscious behavioral patterns, unlike infections like pneumonia or typhoid that may occur inadvertently. Steps such as ensuring blood safety in blood banks, using disposable needles and syringes, promoting condom distribution, controlling drug abuse, advocating safe sex, and regular HIV check-ups in susceptible populations are taken to prevent the spread of HIV infection. It is essential not to hide HIV infection or AIDS as doing so may lead to further transmission. HIV/AIDS-infected individuals deserve help and sympathy from society instead of being stigmatized. Recognizing HIV/AIDS as a collective problem and taking collaborative actions between society and the medical fraternity are crucial in preventing the widespread dissemination of the disease. It requires a joint effort to tackle this malady and prevent its spread.

What enzyme assists in replicating the viral RNA genome of HIV into viral DNA within macrophages?

Reverse transcriptase

The correct answer is Option (2)-Reverse transcriptase 

Reverse transcriptase is the enzyme that assists in replicating the viral RNA genome of HIV into viral DNA within macrophages. This enzyme is unique to retroviruses like HIV, which have RNA as their genetic material. The process involves the reverse transcription of RNA into DNA, which is then integrated into the host cell's genome, allowing the virus to replicate and produce new viral particles. The other enzymes listed have different roles:

• DNA polymerase: Typically involved in the replication of DNA in cellular organisms.
• RNA polymerase: Involved in the transcription of DNA into RNA in cellular organisms.
• Integrase: An enzyme used by HIV to integrate its newly formed viral DNA into the host cell's DNA after reverse transcription has occurred.