Which one of the following enzymes is not used as a tool in recombinant DNA technology?
Answer & explanation
Correct answer: option 2
The correct answer is Option (2) → Lipase
Recombinant DNA technology needs a few key tools: restriction enzymes to cut DNA, polymerase enzymes to copy it, and ligases to join it, along with vectors and a host.
(1) Ligase — A tool of recombinant DNA technology. DNA ligase joins the cut vector and the gene of interest.
(2) Lipase — Not a tool of recombinant DNA technology. It breaks down fats (lipids). It does not act on DNA.
(3) Polymerase — A tool of recombinant DNA technology. A DNA polymerase makes copies of the gene, for example in PCR.
(4) Restriction enzyme — A tool of recombinant DNA technology. It cuts DNA at a specific base sequence.
Note: A similar question was also asked in CUET (UG) 2026 on 24-05-2026 (Slot 1).
NCERT Extract:
Tools of Recombinant DNA Technology – …
