Which of the following techniques is used in the test-tube baby program?

ZIFT(zygote intra fallopian transfer )

The correct answer is Option (3)- ZIFT(zygote intra fallopian transfer )

In vitro fertilisation (IVF–fertilisation outside the body in almost similar conditions as that in the body) followed by embryo transfer (ET) is one of such methods. In this method, popularly known as test tube baby programme, ova from the wife/donor (female) and sperms from the husband/donor (male) are collected and are induced to form zygote under simulated conditions in the laboratory. The zygote or early embryos (with upto 8 blastomeres) could then be transferred into the fallopian tube (ZIFT–zygote intra fallopian transfer) and embryos with more than 8 blastomeres, into the uterus (IUT – intra uterine transfer), to complete its further development.

Transfer of an ovum collected from a donor into the fallopian tube (GIFT – gamete intra fallopian transfer) of another female who cannot produce one, but can provide suitable environment for fertilisation and further development is another method attempted.

IUI: In this technique, the semen collected either from the husband or a healthy donor is artificially introduced either into the vagina or into the uterus (IUI – intra-uterine insemination) of the female.