Practicing Success

Target Exam

CUET

Subject

Biology

Chapter

Biotechnology -Principles and Processes

Question:

Match the Column 1 with Column 2 :

Column 1 Column 2
1) Plasmids  a) Virus infecting bacteria
2) Bacteriophages b) Natural polymer extracted from sea weeds
3) Exonucleases c) Remove nucleotides from the ends of the DNA
4) Agarose  d) Circular extrachromosomal DNA 

 

Options:

1-d,2-a,3-b,4-c

1-d,2-c,3-a,4-b

1-a,2-d,3-c,4-b

1-d,2-a,3-c,4-b

Correct Answer:

1-d,2-a,3-c,4-b

Explanation:

The correct answer is Option (4) -1-d,2-a,3-c,4-b

Column 1 Column 2
1) Plasmids  d) Circular extrachromosomal DNA 
2) Bacteriophages a) Virus infecting bacteria
3) Exonucleases c) Remove nucleotides from the ends of the DNA
4) Agarose  b) Natural polymer extracted from sea weeds

In genetic engineering, a vector is a DNA molecule used to carry and transfer foreign DNA into a host cell. Plasmids are one of the common types of vectors used in genetic engineering experiments. They are small, circular DNA molecules that exist naturally in bacterial cells and can replicate independently of the chromosomal DNA. Plasmids are highly versatile and can be manipulated in the laboratory to carry specific DNA sequences of interest.

Agarose is a natural polymer extracted from sea weeds and is commonly used as a matrix in gel electrophoresis to separate DNA fragments.

Bacteriophages are viruses that infect bacteria and can be used as vectors to transfer foreign DNA into bacterial cells during genetic engineering experiments.Plasmids and bacteriophages are commonly used as vectors in recombinant DNA technology due to their ability to replicate independently within bacterial cells, separate from the chromosomal DNA.

Restriction enzymes belong to a larger class of enzymes called nucleases. These are of two kinds; exonucleases and endonucleases. Exonucleases remove nucleotides from the ends of the DNA whereas, endonucleases make cuts at specific positions within the DNA.