Arrange the following steps involved in Recombinant DNA Technology in correct sequence.
(A) Insertion of recombinant DNA into host cell/organism
(B) Cutting of source DNA as well as vector DNA at specific location and their joining
(C) Isolation of the genetic material (DNA)
(D) Obtaining the foreign gene product
(E) Culturing transformed host cells at large scale
Choose the correct answer from the options given below:
Answer & explanation
Correct answer: option 1
The correct answer is Option (1) → (C), (B), (A), (E), (D)
DNA is first isolated (C), then source and vector DNA are cut and joined (B), the recombinant DNA is put into the host (A), the transformed cells are cultured on a large scale (E), and the foreign gene product is obtained (D).
NCERT Extract:
Processes of Recombinant DNA Technology – “Recombinant DNA technology involves several steps in specific sequence such as isolation of DNA, fragmentation of DNA by restriction endonucleases, isolation of a desired DNA fragment, ligation of the DNA fragment into a vector, transferring the recombinant DNA into the host, culturing the host cells in a medium at large scale and extraction of the desired product.”