The cell wall of a bacteria can be removed by treated it with enzyme :

The correct answer is Option (1) → Lysozyme

To isolate DNA, the cell wall is first broken down with an enzyme that suits the kind of wall.

Option (1) Lysozyme — Correct. To release DNA, the cell wall must be broken. Bacterial cells are treated with lysozyme, which digests the bacterial cell wall; cellulase is used for plant cells and chitinase for fungi.

Option (2) Cellulase — Incorrect. Cellulase breaks the cellulose wall of plant cells.

Option (3) Chitinase — Incorrect. Chitinase breaks the chitin wall of fungi.

Option (4) Pectinase — Incorrect. Pectinase breaks down pectin; it is used to clear fruit juices, not to open bacterial cells.

NCERT Extract:
Isolation of the Genetic Material (DNA) – “… Since the DNA is enclosed within the membranes, we have to break the cell open to release DNA along with other macromolecules such as RNA, proteins, polysaccharides and also lipids. This can be achieved by treating the bacterial cells/plant or animal tissue with enzymes such as lysozyme (bacteria), cellulase (plant cells), chitinase (fungus).”