Select the incorrect statements from the following :

A-Restriction enzymes are used in isolation of DNA from other macro-molecules.

B-Disarmed pathogen vectors are also used in transfer of R-DNA into the host.

C- Retroviruses in plants have the ability to transform normal cells into cancerous cells.

D- The first restriction endonuclease is Hind II .

Choose the correct answer from the given options .

A and C

The correct answer is Option (3) - A and C

A. Restriction enzymes are the enzymes produced by certain bacteria that have the property of cleaving DNA molecule at or near specific base sequences. So, it is used in isolation of a part of DNA from the whole DNA macromolecule. It is not used to isolate DNA from other macromolecules.

C. Agrobacterium tumifaciens, a pathogen of several dicot plants is able to deliver a piece of DNA known as ‘T-DNA’ to transform normal plant cells into a tumor and direct these tumor cells to produce the chemicals required by the pathogen. Similarly, retroviruses in animals have the ability to transform normal cells into cancerous cells.

The correct statements are : B and D

The first restriction endonuclease is Hind II. It was found that Hind II always cut DNA molecules at a particular point by recognising a specific sequence of six base pairs.

The disarmed pathogen’ vectors, which when allowed to infect the cell, transfer the recombinant DNA into the host.