Match the LIST-I with LIST-II
| LIST-I | LIST-II |
|---|---|
| Tools of recombinant DNA technology | Features |
| A. Gel electrophoresis | I. DNA amplification |
| B. PCR | II. DNA separation |
| C. Vector | III. DNA replication |
| D. Host cell | IV. Gene transfer |
Choose the correct answer from the options given below:
Answer & explanation
Correct answer: option 1
The correct answer is Option (1) → A-II, B-I, C-IV, D-III
(A) Gel electrophoresis — this matches (II) DNA separation. DNA fragments carry a negative charge, so they move towards the positive electrode in an electric field. The gel sieves them, and smaller fragments travel farther. The fragments are thus separated by size.
(B) PCR — this matches (I) DNA amplification. In the polymerase chain reaction, repeated cycles with primers and a thermostable DNA polymerase make a very large number of copies of the gene of interest.
(C) Vector — this matches (IV) Gene transfer. A vector, such as a plasmid, is a carrier DNA. The gene of interest is joined to it, and it takes the gene into the host cell.
(D) Host cell — this matches (III) DNA replication. The recombinant DNA is put into a host cell, such as E. coli. The enzymes of the host copy it, so it replicates along with the host’s own DNA.