Which among the following is used to transfer the recombinant DNA into the host ? |
Micro-injection . Biolistics or gene gun . Disarmed pathogen vectors . All of these |
All of these |
a-Micro-injection . b-Biolistics or gene gun. c-Disarmed pathogen vectors. d-All of these is the correct option. In a method known as micro-injection, recombinant DNA is directly injected into the nucleus of an animal cell. In another method, suitable for plants, cells are bombarded with high velocity micro-particles of gold or tungsten coated with DNA in a method known as biolistics or gene gun. And the last method uses ‘disarmed pathogen’ vectors, which when allowed to infect the cell, transfer the recombinant DNA into the host. |