Practicing Success
Practicing Success
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| Which among the following is the correct order of Polymerase Chain Reaction (PCR) ? |
Extension ,denaturation , primer annealing . Denaturation ,extension, primer annealing . Primer annealing, denaturation ,extension . Denaturation ,primer annealing ,extension . |
| Denaturation ,primer annealing ,extension . |
| PCR stands for Polymerase Chain Reaction. In this reaction, multiple copies of the gene (or DNA) of interest is synthesised in vitro using two sets of primers and the enzyme DNA polymerase. Polymerase chain reaction (PCR) : Each cycle has three steps: (i) Denaturation (ii) Primer annealing (iii) Extension of primers |
