Practicing Success

Target Exam

CUET

Subject

Biology

Chapter

Biotechnology -Principles and Processes

Question:

Assertion: Restriction enzymes cut the strand of DNA to produce sticky ends.
Reason: Stickiness of the ends facilitates the action of the enzyme DNA polymerase.

Options:

Both Assertion (A) and Reason (R) are correct and R is the correct explanation of A.

Both Assertion (A) and Reason(R) are correct  but R is not the correct explanation of A.

Assertion (A) is true but Reason (R) is not correct.

Both Assertion (A) and Reason are false (F ).

Correct Answer:

Assertion (A) is true but Reason (R) is not correct.

Explanation:

Restriction enzyme, a type of endonuclease, functions by “inspecting” the length of a DNA sequence. Once it finds a recognition sequence, it binds and cut each of the two strands of the double helix at specific point leaving single stranded portions at the ends. This results in overhanging stretches called sticky ends. These are named so because they form hydrogen bonds with their complementary counter parts i.e., they can join similar complementary ends of DNA fragment from some other source with the help of DNA ligase. This stickness of the ends facilitates the action of the enzyme DNA ligase, not DNA polymerase.