Read the following passage and answer the questions that follow:- |
Which of the following statement is correct? |
Taq polymerase, which is isolated from bacterium Thermus aquaticus is stable at low temperature only. With the help of DNA helicase, the complementary sticky ends of the DNA are joined to produce rDNA. The RNA can be removed by treatment with ribonuclease whereas proteins can be removed by treatment with protease. None of these |
The RNA can be removed by treatment with ribonuclease whereas proteins can be removed by treatment with protease. |
The correct answer is Option (3) -DNA purified from the cell is precipitated by adding chilled ethanol. The incorrect Statements can be corrected as : Taq polymerase, which is isolated from bacterium Thermus aquaticus is stable at high temperatures also(95oC). The correct statement is : The RNA can be removed by treatment with ribonuclease whereas proteins can be removed by treatment with protease. Other molecules can be removed by appropriate treatments and purified DNA ultimately precipitates out after the addition of chilled ethanol. |