Which of the following is not considered a tool of recombinant DNA Technology?

Downstream processing

The correct answer is Option (2) →Downstream processing

Downstream processing is not considered a tool of recombinant DNA technology; it refers to the series of processes used to purify and process the product after the recombinant DNA technology has been applied.

Other options are tools used in recombinant DNA technology:

• Restriction enzymes: These enzymes cut DNA at specific sites, making it possible to isolate and manipulate DNA segments.
• Cloning vectors: These are DNA molecules (like plasmids) that carry foreign DNA into a host cell for replication and expression.

Competent host: A competent host is a host that allows easy insertion of the cloning vector inside it and, on the other hand, produces positive results as required by the scientist.

Therefore, downstream processing is not a tool used in the creation of recombinant DNA but rather a step that comes after the genetic engineering process.