Target Exam

CUET

Subject

Biology

Chapter

Biotechnology -Principles and Processes

Question:

The protein formed by the encoded gene expression in a heterologous host is called:

Options:

Structural protein

Recombinant protein

Transposone

Prohormone

Correct Answer:

Recombinant protein

Explanation:

The correct answer is Option (1) –Recombinant protein

When you insert a piece of alien DNA into a cloning vector and transfer it into a bacterial, plant or animal cell, the alien DNA gets multiplied. In almost all recombinant technologies, the ultimate aim is to produce a desirable protein. Hence, there is a need for the recombinant DNA to be expressed. After having cloned the gene of interest and having optimised the conditions to induce the expression of the target protein, one has to consider producing it on a large scale.

If any protein encoding gene is expressed in a heterologous host, it is called a recombinant protein. The cells harbouring cloned genes of interest may be grown on a small scale in the laboratory. The cultures may be used for extracting the desired protein and then purifying it by using different separation techniques.