Practicing Success

Target Exam

CUET

Subject

Biology

Chapter

Biotechnology -Principles and Processes

Question:
Which among the following statements about cloning vectors is incorrect ?
Options:
To link the alien DNA, the vector needs to have a single recognition site for the commonly used restriction enzymes.
‘Ori’ is a sequence responsible for controlling the copy number of the linked DNA.
Selectable marker selectively permitting the growth of the non-transformants .
The ligation of alien DNA is carried out at a restriction site present in one of the two antibiotic resistance genes.
Correct Answer:
Selectable marker selectively permitting the growth of the non-transformants .
Explanation:
In addition to ‘ori’, the vector requires a selectable marker, which helps in identifying and eliminating non-transformants and selectively permitting the growth of the transformants.