Which Mughal Emperor was defeated by Sher Shah Sur?

Humayun

The correct answer is Option (3) → Humayun

Babur's successor, Nasiruddin Humayun (1530-40, 1555-56) expanded the frontiers of the empire, but lost it to the Afghan leader Sher Shah Sur, who drove him into exile. Humayun took refuge in the court of the Safavid ruler of Iran. In 1555 Humayun defeated the Surs, but died a year later.