Match List-I with List-II
| List-I Enzymes | List-II Used for |
|---|---|
| (A). Protease | (I). RNA |
| (B). Chitinase | (II). Protein |
| (C). Lysozyme | (III). Fungus |
| (D). Ribonuclease | (IV). Bacterial cell |
Choose the correct answer from the options given below:
Answer & explanation
Correct answer: option 1
The correct answer is Option (1) → (A)-(II), (B)-(III), (C)-(IV), (D)-(I)
(A) Protease — this matches (II) Protein. Proteins such as histones are bound to the DNA. Protease breaks down these proteins, so they can be removed and the DNA comes out pure.
(B) Chitinase — this matches (III) Fungus. The cell wall of a fungus is made of chitin. Chitinase digests chitin, so the fungal cell opens and its DNA is released.
(C) Lysozyme — this matches (IV) Bacterial cell. Lysozyme breaks down the bacterial cell wall. This is the first step in taking DNA out of a bacterium.
(D) Ribonuclease — this matches (I) RNA. RNA is present in the cell along with the DNA. Ribonuclease breaks down the RNA, so only the DNA is left.
NCERT Extract:
Isolation of the Genetic Material (DNA) – “… Since the DNA is enclosed within the membranes, we have to break the cell open to release DNA along with other macromolecules such as RNA, proteins, polysaccharides and also lipids. This can be achieved by treating the bacterial cells/plant or animal tissue with enzymes such as lysozyme (bacteria), cellulase (plant cells), chitinase (fungus). You know that genes are located on long molecules of DNA interwined with proteins such as histones. The RNA can be removed by treatment with ribonuclease whereas proteins can be removed by treatment with protease.”