Which company prepared human insulin in 1983?

The correct answer is Option (2) → Eli Lilly

Insulin is made in the body as a pro-hormone with an extra stretch, the C peptide, which must be removed. Making mature insulin by rDNA (recombinant DNA) technology was therefore hard. In 1983, Eli Lilly, an American company, prepared two DNA sequences for the A and B chains of human insulin and put them into plasmids of E. coli. The two chains were made separately, extracted and joined by disulfide bonds to form human insulin.

NCERT Extract:
Genetically Engineered Insulin – “… The main challenge for production of insulin using rDNA techniques was getting insulin assembled into a mature form. In 1983, Eli Lilly an American company prepared two DNA sequences corresponding to A and B, chains of human insulin and introduced them in plasmids of E. coli to produce insulin chains. Chains A and B were produced separately, extracted and combined by creating disulfide bonds to form human insulin.”