Practicing Success

Target Exam

CUET

Subject

Biology

Chapter

Biotechnology -Principles and Processes

Question:

Statement I: The tumour inducing plasmid (Ti plasmid) of Agrobacterium tumefaciens acts as a cloning vector in recombinant DNA technology.

Statement II: The Ti plasmid which is used in the mechanisms of delivering genes to a cell remains pathogenic.

 

Options:

Only statement I is correct .

Only statement II is correct .

Both statements I and II are correct .

Both statements I and II are incorrect .

Correct Answer:

Only statement I is correct .

Explanation:

The correct answer is Option (1) - Only statement I is correct .

Statement I: The tumour-inducing plasmid (Ti plasmid) of Agrobacterium tumefaciens can be used as a cloning vector in recombinant DNA technology.This is correct.

Statement II: The Ti plasmid which is used in the mechanisms of delivering genes to a cell remains pathogenic.This is incorrect. Although the Ti plasmid is utilized for delivering genes to plant cells in recombinant DNA technology, it has been modified to eliminate its pathogenicity, making it safe for use as a vector.

In recombinant DNA technology, the tumour-inducing plasmid (Ti plasmid) of Agrobacterium tumefaciens is utilized as a cloning vector. This plasmid is capable of delivering foreign genes into plant cells, enabling the genetic modification of plants. By incorporating the desired gene of interest into the Ti plasmid, it can be introduced into plant cells, where it can integrate into the plant genome and lead to the expression of the introduced gene. However, during the process of using the Ti plasmid as a cloning vector, it is modified to eliminate its pathogenicity, ensuring that it does not cause tumour formation in the plants or any harm to the host organisms. The modified Ti plasmid serves as a safe and efficient tool for introducing desirable genes into plants through genetic engineering techniques.