Which of the following is not the correctly matched pair of organism…

The correct answer is Option (2) → Algae - Methylase

Option (1) Fungi-Chitinase — A correctly matched pair. The fungal cell wall is made of chitin, and chitinase breaks it down.

Option (2) Algae - Methylase — Not a correctly matched pair. A methylase adds methyl groups to DNA. In bacteria it protects the cell’s own DNA from its restriction enzymes. It does not break down any cell wall.

Option (3) Plant cells-Cellulase — A correctly matched pair. The plant cell wall is made mainly of cellulose, which cellulase digests.

Option (4) Bacteria-Lysozyme — A correctly matched pair. Lysozyme breaks down the bacterial cell wall, so the cell bursts and releases its DNA.

NCERT Extract:
Isolation of the Genetic Material (DNA) – “… Recall that nucleic acid is the genetic material of all organisms without exception. In majority of organisms this is deoxyribonucleic acid or DNA. In order to cut the DNA with restriction enzymes, it needs to be in pure form, free from other macro-molecules. Since the DNA is enclosed within the membranes, we have to break the cell open to release DNA along with other macromolecules such as RNA, proteins, polysaccharides and also lipids. This can be achieved by treating the bacterial cells/plant or animal tissue with enzymes such as lysozyme (bacteria), cellulase (plant cells), chitinase (fungus).”
Restriction Enzymes – “In the year 1963, the two enzymes responsible for restricting the growth of bacteriophage in Escherichia coli were isolated. One of these added methyl groups to DNA, while the other cut DNA. The later was called restriction endonuclease.”