Target Exam

CUET

Subject

Biology

Chapter

Biotechnology -Principles and Processes

Question:

Match the Enzyme in List - I with its Activity in List - II.

List – I

List – II

(A) Taq DNA Polymerase

(I) Cleaves the DNA at ends

(B) Exonuclease

(II) Breaks the fungal cell wall

(C) Protease

(III) Stable above 90°C

(D) Chitinase

(IV) Break the protein molecules

Choose the correct answer from the options given below:

Options:

(A)-(III), (B)-(IV), (C)-(I), (D)-(II)

(A)-(III), (B)-(II), (C)-(IV), (D)-(I)

(A)-(III), (B)-(I), (C)-(IV), (D)-(II)

(A)-(II), (B)-(I), (C)-(III), (D)-(IV)

Correct Answer:

(A)-(III), (B)-(I), (C)-(IV), (D)-(II)

Explanation:

The correct answer is Option (3) → (A)-(III), (B)-(I), (C)-(IV), (D)-(II)

List – I

List – II

(A) Taq DNA Polymerase

(III) Stable above 90°C

(B) Exonuclease

(I) Cleaves the DNA at ends

(C) Protease

(IV) Break the protein molecules

(D) Chitinase

(II) Breaks the fungal cell wall

A. Taq polymerase is a thermostable DNA polymerase derived from the bacterium Thermus aquaticus which remain active during the high temperature induced denaturation of double stranded DNA. It is commonly used in the polymerase chain reaction (PCR) to extend primers and amplify DNA segments.


B. Restriction enzymes belong to a larger class of enzymes called nucleases. These are of two kinds; exonucleases and endonucleases. Exonucleases remove nucleotides from the ends of the DNA whereas, endonucleases make cuts at specific positions within the DNA.

C. Protease enzyme breaks down proteins by hydrolyzing peptide bonds. While proteases are important for many biological processes, they do not break down cellulose and therefore are not used to degrade plant cell walls.

D. Fungal cell walls contain chitin, a strong and flexible polymer. Chitinase is an enzyme that breaks down chitin, making it effective in disrupting the cell walls of fungi.