Target Exam

CUET

Subject

Biology

Chapter

Molecular Basis of Inheritance

Question:

Read the passage carefully and answer the given questions.

DNA fingerprinting

Polymorphism in DNA sequence is the basis of genetic mapping of the human genome as well as of DNA fingerprinting. DNA fingerprinting involves identification of differences in some specific regions in the DNA sequence called repetitive DNA. It works on the principle of polymorphisms in DNA sequences. The repetitive DNA are separated from bulk genomic DNA. The bulk DNA forms a major peak and the other small peaks are referred to as satellite DNA. These repetitive/satellite DNA sequences show a high degree of polymorphisms and form the basis of DNA fingerprinting.

Variable Number of Tandem Repeats are -

Options:

Satellite DNA

Silent DNA

SNPs

ESTs

Correct Answer:

Satellite DNA

Explanation:

The correct answer is Option (1) → Satellite DNA  

DNA fingerprinting involves identifying differences in some specific regions in DNA sequence called as repetitive DNA, because in these sequences, a small stretch of DNA is repeated many times.The technique of DNA Fingerprinting was initially developed by Alec Jeffreys. He used a satellite DNA as probe that shows very high degree of polymorphism. It was called a Variable Number of Tandem Repeats (VNTR). The technique, as used earlier, involved Southern blot hybridisation using radiolabelled VNTR as a probe.