The most number of nucleotides will be present in (from left to right)

The correct answer is Option (4) → Lane 1, band 1

Larger DNA fragments move more slowly and stay nearest the wells. Lane 1, band 1 lies closest to the well, so it is the largest fragment with the most nucleotides.

Option (1) Lane 4, band 6 — Incorrect. A band far from the well is a small fragment.

Option (2) Lane 2, band 2 — Incorrect. This band has moved further than lane 1, band 1.

Option (3) Lane 3, band 4 — Incorrect. This band has moved further than lane 1, band 1.

Option (4) Lane 1, band 1 — Correct. In gel electrophoresis, DNA fragments separate by size: smaller fragments move farther through the gel and larger ones stay near the well. Lane 1, band 1 is the band nearest the well, so it has the largest fragment, with the most nucleotides.