Answer the following questions from the diagram given below:-

The most number of nucleotides will be present in (from left to right)

Lane 1, band 1

The correct answer is Option (4) → Lane 1, band 1

Gel electrophoresis is a technique used to separate macromolecules based on their size and charge. DNA molecules, which carry a negative charge due to the phosphate groups in their nucleotides, will move towards the positive electrode (anode) when placed in the wells near the negative electrode (cathode) of the gel. During the electrophoresis process, smaller DNA fragments travel farther from the negative electrode, while larger fragments move more slowly and remain closer to it.

To determine which band has the most number of nucleotides during gel electrophoresis, we need to consider the size of the DNA fragments, which is inversely proportional to their migration distance. The band that is closest to the cathode (negative electrode) will have the largest DNA fragments, and thus, the most nucleotides.

In the provided image, the DNA fragment in band 1 is nearest to the cathode, indicating that it is the largest in size. The distinct positions of all the bands suggest that the DNA fragments vary in size. The fragment in band 5, being the farthest from the negative terminal, has traveled the greatest distance, signifying that it is the smallest in size.

The correct answer will be Option 4: Lane 1, band 1, assuming this band is closest to the cathode (negative terminal). This band would contain the largest DNA fragment, meaning it has the most nucleotides.