The key tools of recombinant DNA technology are:
(A) Ligases
(B) Restriction enzymes
(C) Polymerase enzymes
(D) Suitable vectors and host organisms
Choose the correct answer from the options given below:
Answer & explanation
Correct answer: option 3
The correct answer is Option (3) → (A), (B), (C) and (D)
Recombinant DNA technology joins a desired gene to a carrier DNA and puts it into a host, so that the host makes many copies of it or its product. Each step needs a tool.
(A) Ligases Correct. DNA ligase joins the cut ends of DNA. It seals the gene of interest into the vector and so forms the recombinant DNA.
(B) Restriction enzymes Correct. These are the ‘molecular scissors’. They cut DNA at specific recognition sequences, so the gene and the vector get matching sticky ends.
(C) Polymerase enzymes Correct. DNA polymerase makes many copies of the gene. In PCR, the heat-stable Taq polymerase is used for this.
(D) Suitable vectors and host organisms Correct. A vector, such as a plasmid, carries the gene into the host cell. The host, such as E. coli, multiplies the recombinant DNA and makes the gene’s product.
Note: A similar question was also asked in CUET (UG) 2023 on 27-05-2023 (Slot 3).
NCERT Extract:
Tools of Recombinant DNA Technology – …
