Target Exam

CUET

Subject

Biology

Chapter

Biotechnology and its Applications

Question:

In RNA interference method, nematode specific genes were introduced into the host plant which produce _________.

Options:

only sense strand of RNA in the host

only antisense strand of RNA in the host

both sense and antisense strands of RNA in the host

dsDNA for cellular defense

Correct Answer:

both sense and antisense strands of RNA in the host

Explanation:

The correct answer is Option (3) → both sense and antisense strands of RNA in the host

A nematode Meloidegyne incognitia infects the roots of tobacco plants and causes a great reduction in yield. A novel strategy was adopted to prevent this infestation which was based on the process of RNA interference (RNAi).Using Agrobacterium vectors, nematode-specific genes were introduced into the host plant . The introduction of DNA was such that it produced both sense and anti-sense RNA in the host cells. These two RNA’s being complementary to each other formed a double stranded (dsRNA) that initiated RNAi and thus, silenced the specific mRNA of the nematode.