In RNA interference method, nematode specific genes were introduced into the host plant which produce _________. |
only sense strand of RNA in the host only antisense strand of RNA in the host both sense and antisense strands of RNA in the host dsDNA for cellular defense |
both sense and antisense strands of RNA in the host |
The correct answer is Option (3) → both sense and antisense strands of RNA in the host A nematode Meloidegyne incognitia infects the roots of tobacco plants and causes a great reduction in yield. A novel strategy was adopted to prevent this infestation which was based on the process of RNA interference (RNAi).Using Agrobacterium vectors, nematode-specific genes were introduced into the host plant . The introduction of DNA was such that it produced both sense and anti-sense RNA in the host cells. These two RNA’s being complementary to each other formed a double stranded (dsRNA) that initiated RNAi and thus, silenced the specific mRNA of the nematode. |