Enzyme helpful in polymerizing RNA with defined sequences in a template independent manner is:
Answer & explanation
Correct answer: option 2
The correct answer is Option (2) → Polynucleotide phosphorylase
Explanation:
Polynucleotide phosphorylase:
- Severo Ochoa enzyme (polynucleotide phosphorylase) is helpful in polymerising RNA with defined sequences in a template independent manner (enzymatic synthesis of RNA).
- It uses nucleotide diphosphates as substrates and can create RNA with defined sequences without requiring a DNA or RNA template.
Mononucleotide phosphorylase:
- This is not an enzyme involved in RNA polymerization.
RNA polymerase I:
- It is a eukaryotic enzyme that synthesizes rRNA in a template-dependent manner.
DNA-dependent RNA polymerase:
- This enzyme synthesizes RNA based on a DNA template and is involved in transcription.
Key Point:
The distinguishing factor is that Polynucleotide phosphorylase does not require a template, unlike RNA polymerases which are always template-dependent.