Practicing Success
Which term is used to describe the special sequence in DNA recognized by restriction endonucleases? |
Restriction nucleotide sequence Palindromic nucleotide sequence Recognition nucleotide sequence All of the above |
Palindromic nucleotide sequence |
The special sequence in the DNA recognized by restriction endonuclease is called a "palindromic nucleotide sequence." Explanation: Restriction enzymes are part of a larger group of enzymes known as nucleases, which are classified into two kinds: exonucleases and endonucleases. Exonucleases remove nucleotides from the ends of DNA, whereas endonucleases make cuts at specific positions within the DNA. Each restriction endonuclease operates by inspecting the length of a DNA sequence. When it identifies its specific recognition sequence, it binds to the DNA and cuts both strands of the double helix at specific points in their sugar-phosphate backbones. Every restriction endonuclease recognizes a particular palindromic nucleotide sequence in the DNA. Palindromic nucleotide sequences are sequences of DNA that read the same on both strands when read in the 5' to 3' direction. Restriction endonucleases are enzymes that cleave DNA at specific recognition sequences, and many of these sequences are palindromic, meaning they have the same sequence of bases on both the forward and reverse strands. When a restriction endonuclease recognizes a palindromic sequence, it binds to the DNA at that site and cuts both strands of the DNA at specific positions, resulting in the formation of "sticky ends" or "blunt ends," which are important in genetic engineering and molecular biology techniques.
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