Eli Lilly, in 1983, developed human insulin using recombinant DNA Technology by combining chain A and chain B with:
Answer & explanation
Correct answer: option 4
The correct answer is Option (4) → Disulfide bonds
Option (4) Disulfide bonds — Correct. Eli Lilly made the A and B chains of human insulin separately in E. coli. The chains were extracted and joined by creating disulfide bonds, to form human insulin.
NCERT Extract:
Genetically Engineered Insulin – “… In 1983, Eli Lilly an American company prepared two DNA sequences corresponding to A and B, chains of human insulin and introduced them in plasmids of E. coli to produce insulin chains. Chains A and B were produced separately, extracted and combined by creating disulfide bonds to form human insulin.”