Silencing of the gene could be achieved through the use of what?

Both RNAi and antisense RNA

Silencing of a gene can be achieved through the use of both RNAi (RNA interference) and antisense RNA.

RNA interference (RNAi) and antisense RNA are both mechanisms used in molecular biology to silence or inhibit the expression of specific genes.

• RNA interference (RNAi) involves the introduction of double-stranded RNA molecules that are complementary to the target gene's mRNA. This triggers the degradation of the mRNA, preventing its translation into protein, thus silencing the gene expression.
• Antisense RNA is a single-stranded RNA molecule that is complementary to the mRNA sequence of the target gene. When introduced into cells, antisense RNA binds to the mRNA, forming a double-stranded RNA structure. This binding can interfere with the normal processing or translation of the mRNA, leading to the inhibition of gene expression.

Both RNAi and antisense RNA methods can be utilized to silence specific genes and regulate gene expression in experimental settings and potential therapeutic applications.