What happens when an alien DNA is ligated into the tetracycline resistance gene of a vector during gene cloning?

The recombinant will lose tetracycline resistance.

The correct answer is option (2) -The recombinant will lose tetracycline resistance.

When an alien DNA is ligated into the tetracycline resistance gene of a vector, the recombinant will lose tetracycline resistance.

The tetracycline resistance gene in the vector provides the host bacterium with resistance against the antibiotic tetracycline. This resistance allows the bacterial cells to survive and grow in the presence of tetracycline, which is toxic to non-resistant cells.

However, when foreign DNA is inserted into the tetracycline resistance gene during the process of gene cloning, it disrupts the normal function of the gene. As a result, the recombinant bacterium carrying the vector with the inserted alien DNA will lose its ability to resist tetracycline. The recombinant cells will become sensitive to tetracycline and will no longer survive in the presence of this antibiotic.

So, the correct option is:

(2) The recombinant will lose tetracycline resistance.