Identify the tools for recombinant DNA technology:
A. Restriction enzymes and host organism
B. Ligases and vectors
C. Ribosome
D. Polymerase enzyme
Choose the correct answer from the options given below:
Answer & explanation
Correct answer: option 1
The correct answer is Option (1) → A, B and D only
- A. Restriction enzymes and host organism: Restriction enzymes are used to cut DNA at specific sequences, and host organisms are necessary for cloning and expression of recombinant DNA.
- B. Ligases and vectors: Ligases are enzymes that join DNA fragments together, and vectors are DNA molecules used to carry foreign genetic material into a host cell.
- D. Polymerase enzyme: Polymerase enzymes are used for amplifying DNA sequences (e.g., through PCR) and are essential in recombinant DNA technology.
C. Ribosome is not directly involved in recombinant DNA technology; it is primarily involved in protein synthesis.