Identify the tools for recombinant DNA technology:

A. Restriction enzymes and host organism
B. Ligases and vectors
C. Ribosome
D. Polymerase enzyme

Choose the correct answer from the options given below:

A, B and D only

The correct answer is Option (1) → A, B and D only

• A. Restriction enzymes and host organism: Restriction enzymes are used to cut DNA at specific sequences, and host organisms are necessary for cloning and expression of recombinant DNA.
• B. Ligases and vectors: Ligases are enzymes that join DNA fragments together, and vectors are DNA molecules used to carry foreign genetic material into a host cell.
• D. Polymerase enzyme: Polymerase enzymes are used for amplifying DNA sequences (e.g., through PCR) and are essential in recombinant DNA technology.

C. Ribosome is not directly involved in recombinant DNA technology; it is primarily involved in protein synthesis.