Target Exam

CUET

Subject

Biology

Chapter

Biotechnology -Principles and Processes

Question:

Read the following passage carefully.

Restriction enzyme

Restriction enzymes cut the strand of DNA a little away from the center of the palindrome sites, but between the same two bases on the opposite strands. This leaves single stranded portions at the ends. There are overhanging stretches called sticky ends on each strand. These are named so because they form hydrogen bonds with their complementary cut counterparts. This stickiness of the ends facilitates the action of the enzyme DNA ligase. The word palindromes refer to group of letters that forms the same words when read both forward and backward. Restriction endonucleases are used in genetic engineering as molecular scissors.

Palindromes are:

Options:

The same group of letters when read both forward and backward

The different group of letters when read both forward and backward

Same group of letters when read only forward

Same group of letters when read only backward

Correct Answer:

The same group of letters when read both forward and backward

Explanation:

The correct answer is Option (1) → The same group of letters when read both forward and backward   

Restriction Endonucleases (Molecular Scissors): Restriction enzymes belong to a larger class of enzymes called nucleases. These are enzymes that are commonly referred to as "molecular scissors" because they cut DNA molecules at specific recognition sequences. These recognition sequences are often palindromic, meaning the sequence reads the same forwards and backward.

The palindrome in DNA is a sequence of base pairs that reads same on the two strands when orientation of reading is kept the same. For example, the following sequences reads the same on the two strands in 5' → 3' direction. This is also true if read in the 3' → 5' direction.

5' —— GAATTC —— 3'

3' —— CTTAAG —— 5