Target Exam

CUET

Subject

Biology

Chapter

Biotechnology and its Applications

Question:

What is the method of producing thousands of genetically identical plants through tissue culture called?

Options:

Somatic hybridisation

Genetic modification

Micro-propagation

Cross-breeding

Correct Answer:

Micro-propagation

Explanation:

The correct answer is Option (3) -Micro-propagation

As traditional breeding techniques failed to keep pace with demand and to provide sufficiently fast and efficient systems for crop improvement, another technology called tissue culture got developed. It was learnt by scientists, during 1950s, that whole plants could be regenerated from explants, i.e., any part of a plant taken out and grown in a test tube, under sterile conditions in special nutrient media. This capacity to generate a whole plant from any cell/explant is called totipotency.

The nutrient medium must provide a carbon source such as sucrose and also inorganic salts, vitamins, amino acids and growth regulators like auxins, cytokinins etc. By application of these methods it is possible to achieve propagation of a large number of plants in very short durations. This method of producing thousands of plants through tissue culture is called micro-propagation. Each of these plants will be genetically identical to the original plant from which they were grown, i.e., they are somaclones. Many important food plants like tomato, banana, apple, etc., have been produced on commercial scale using this method. Try to visit a tissue culture laboratory with your teacher to better understand and appreciate the process.