Practicing Success
Cohen and Boyer created the first recombinant DNA using plasmid of : |
Escherichia coli Thermus aquaticus Salmonella typhimurium Bacillus thuringiensis |
Salmonella typhimurium |
The correct answer is Option (3) - Salmonella typhimurium The construction of the first recombinant DNA emerged from the possibility of linking a gene encoding antibiotic resistance with a native plasmid (autonomously replicating circular extra-chromosomal DNA) of Salmonella typhimurium. Stanley Cohen and Herbert Boyer accomplished this in 1972 by isolating the antibiotic resistance gene by cutting out a piece of DNA from a plasmid which was responsible for conferring antibiotic resistance.This recombinant DNA was then transferred into Escherichia coli. Thermus aquaticus is a thermostable bacterium whose enzymes are used in PCR. |