Identify the molecular diagnostic technique from the following:

(A) Enzyme Linked Immuno Sorbent Assay
(B) Polymerase Chain Reaction
(C) Cloning
(D) Recombinant DNA Technology

Choose the correct answer from the options given below:

(A), (B) and (D) Only

The correct answer is Option (2) → (A), (B) and (D) Only

Early diagnosis and understanding the pathophysiology of a disease are crucial for effective treatment. However, conventional diagnostic methods like serum and urine analysis often do not facilitate early detection.

To address this limitation, advanced techniques such as Recombinant DNA technology, Polymerase Chain Reaction (PCR), and Enzyme-Linked Immunosorbent Assay (ELISA) play a significant role in achieving early diagnosis and enhancing treatment outcomes.

The molecular diagnostic techniques among the options are:

• (A) Enzyme Linked Immuno Sorbent Assay (ELISA): Used for detecting and quantifying antigens or antibodies in a sample, commonly applied in diagnostic testing.
• (B) Polymerase Chain Reaction (PCR): A technique to amplify DNA sequences, widely used in diagnosing infections and genetic disorders at the molecular level.
• (D) Recombinant DNA Technology: Involves manipulating DNA for various applications, including diagnostics, by producing specific DNA sequences for identifying pathogens or genetic mutations.

(C) Cloning is not primarily a diagnostic technique but rather a method of creating identical copies of organisms or cells.