Read the following passage carefully. Restriction enzyme Restriction enzymes cut the strand of DNA a little away from the center of the palindrome sites, but between the same two bases on the opposite strands. This leaves single stranded portions at the ends. There are overhanging stretches called sticky ends on each strand. These are named so because they form hydrogen bonds with their complementary cut counterparts. This stickiness of the ends facilitates the action of the enzyme DNA ligase. The word palindromes refer to group of letters that forms the same words when read both forward and backward. Restriction endonucleases are used in genetic engineering as molecular scissors. |
Which one of the following option is correct about restriction enzyme? |
It helps in binding of DNA. This is known as molecular scissors. It has ability to multiply copies of plasmid. It helps in transfer of piece of DNA. |
This is known as molecular scissors. |
The correct answer is Option (2) → This is known as molecular scissors. Restriction Endonucleases (Molecular Scissors): Restriction enzymes belong to a larger class of enzymes called nucleases. These are enzymes that are commonly referred to as "molecular scissors" because they cut DNA molecules at specific recognition sequences. These recognition sequences are often palindromic, meaning the sequence reads the same forwards and backward. |