Match List-I with List-II.

Choose the correct answer from the options given below:

(A)-(IV), (B)-(III), (C)-(II), (D)-(I)

A.Whole plants could be regenerated from explants, i.e., any part of a plant taken out and grown in a test tube, under sterile conditions in special nutrient media.

B.By application of these methods it is possible to achieve propagation of a large number of plants in very short durations. This method of producing thousands of plants through tissue culture is called micro-propagation. Each of these plants will be genetically identical to the original plant from which they were grown, i.e., they are somaclones.

C.Another important application of the method is the recovery of healthy plants from diseased plants. Although the plant is infected with a virus, the meristem (apical and axillary) is free of virus. Hence, one can remove the meristem and grow it in vitro to obtain virus-free plants.

D.Isolated protoplasts from two different varieties of plants – each having a desirable character – can be fused to get hybrid protoplasts, which can be further grown to form a new plant. These hybrids are called somatic hybrids while the process is called somatic hybridisation. Imagine a situation when a protoplast of tomato is fused with that of potato, and then they are grown – to form new hybrid plants combining tomato and potato characteristics. Well, this has been achieved – resulting in formation of pomato.